Product Name :
p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88]
Predicted band size :
53 kDa
Observed band size :
53 kDa
Synonyms:
Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody 展开
Function :
p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within human p53 (acetyl K382) aa 344-393.
Species reactivity:
Human
Verify application:
WB, IF-Cell
Coupling :
unconjugated
Clone number:
PSH06-88
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.44864-47-3 Data Sheet Aliquot store at -20℃.6,6′-Dibromo-2,2′-bipyridyl Chemscene Avoid repeated freeze / thaw cycles.PMID:33733470
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 53 kDa
Positive control :
HepG2 starved overnight treated with 30μg/mL etoposide for 7 hours then add 500ng/mL TSA for 3 hours cell lysate, HeLa cells treated with 500ng/mL TSA for 4 hours.